BactoReal® Nocardia spp.
Background: Nocardia is a genus of Gram-positive, facultative intracellular bacteria belonging to the Actinomycetes. Nocardia species are found worldwide in soil, water and plants. This genus has a total of 85 species, of these > 25 have been recognized as causes of human infections, and > 30 are responsible for animal diseases. Nocardia spp. cause opportunistic disease in humans and animals. Nocardiosis is an infectious disease affecting either the lungs, but it may spread to other organ systems, most often the brain and the skin. It may also involve the kidneys, joints, heart, eyes, and bones. Bovine mastitis is the most common clinical manifestation of nocardiosis among domestic ruminants. Nocardial mastitis is observed in dairy herds submitted to inadequate milking management and/or poor hygienic conditions in pre- and post-milking. The most important pathogenic species for humans and animals are N. asteroides complex, N. brasiliensis, N. pseudobrasiliensis, N. otitidiscaviarum and N. transvalensis.
BactoReal® Nocardia spp. is a detection assay for rapid and sensitive detection of DNA of Nocardia spp. by real-time PCR. This assay has an analytical sensitivity of 50 copies/reaction, as in negative samples the presence of contaminating DNA of Actinomycetes in the extraction or amplification reagents results in weak positive amplification. It may also detect other Actinomycetes.
Specificity and sensitivity: See product description
Also available as kitversion: BactoReal Kit Nocardia spp.
| Product | BactoReal® Nocardia spp. |
| Order no. | RTGV909 |
| Unit | 100 reactions |
| Technology | Real-time PCR |
| Target gene | 16S rDNA gene, detection in FAM channel |
| Content | Assay (pimers + probe) for Nocardia spp. Positive Control Nocardia |
| Amplification mix & IPC | Not included, see product description |
| PCR-Platforms | Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments. |
Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– ControlReal tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
– Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).
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