ViroReal® Bovine Coronavirus

 

ViroReal Bovine CoronavirusViroReal Assay FAM VET RNA mit Gefahrensymbol

 

 

Background: Bovine coronavirus (BCoV) is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae. The BCoV virion is enveloped and spherical in shape. The genome is a single-stranded, positive-sense RNA molecule. BCoV causes diarrhea in adult cattle (Winter Dysentery) and wild ruminants, as well as enteric and respiratory diseases in calves. Winter Dysentery is characterized by high morbidity (50-100%) and low mortality (1-2%), the highest incidence is between November and April when cattle are kept in close confinement. In calves morbidity and mortality are high. BCoV has been found in cattle worldwide.

ViroReal® Bovine Coronavirus is a detection assay for rapid and sensitive detection of RNA of bovine coronavirus (BCoV) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Bovine Coronavirus

Product ViroReal® Bovine Coronavirus
Order no. RTGV20
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene N gene, detection in FAM channel
Content Assay (pimers + probe) for Bovine Coronavirus
Positive Control Bovine Coronavirus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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