ViroReal® Bovine Herpesvirus 1

 

ViroReal Bovine Herpesvirus I

 

 

Background: Bovine herpesvirus 1 (BHV-1) is a DNA virus of the family Herpesviridae, subfamily Alphaherpesvirinae. BHV-1 can cause both clinical and subclinical infections in cattle, including infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IBV) and infectious balanoposthitis (IBP). BHV-1 causes a lifelong latent infection in the trigeminal or sacral ganglia. Animals with a latent BHV-1 infection may serve as a source of infection for susceptible animals. BHV-1 is transmitted mainly through respiratory, ocular or genital secretions.

ViroReal® Bovine Herpesvirus 1 is a detection assay for rapid and sensitive detection of DNA of the bovine herpesvirus 1 (BHV-1) by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Bovine Herpesvirus 1

Product ViroReal® Bovine Herpesvirus 1
Order no. RTGV28
Unit 100 reactions
Technology Real-time PCR
Target gene UL27, detection in FAM channel
Content Assay (pimers + probe) for BHV-1
Positive Control BHV-1
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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