ViroReal® Bovine Nebovirus

 

ViroReal Bovine Nebovirus

 

Background: Neboviruses are non-enveloped, single-stranded, linear, positive-sense RNA viruses ((+)ssRNA), belonging to the Caliciviridae family. The only currently known species in the genus Nebovirus is Newbury-1 virus. Cattle, especially calves, serve as natural hosts. Neboviral infection is associated with gastrointestinal disorders and necrotizing hepatitis leading to fatal hemorrhages in calves. The disease often remains endemic due to the lack of a commercially available vaccine.

ViroReal® Bovine Nebovirus is a detection assay for rapid and sensitive detection of RNA of Newbury-1 virus by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Bovine Nebovirus

Product ViroReal® Bovine Nebovirus
Order no. RTGV23
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene ORF1 , detection in FAM channel
Content Assay (pimers + probe) for bovine nebovirus
Positive Control bovine nebovirus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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