ViroReal® Bovine Norovirus

 

ViroReal Bovine Norovirus

 

Background: Noroviruses are non-enveloped, single-stranded, positive-sense RNA viruses ((+)ssRNA), belonging to the family Caliciviridae. Strains are classified in 5 genotypes (GI ÔÇô GV), infecting different species, including human, bovine, swine, mice and oysters. Bovine norovirus belong to GIII. Norovirus GIII includes two genotypes (GIII.1 and GIII.2), with genotype GIII.1 represented by Jena virus and GIII.2 represented by Newbury-2 virus. Infection is associated with gastrointestinal manifestations such as diarrhea, villus atrophy and crypt hyperplasia of the proximal small intestine in cattle, especially in young calves.

ViroReal® Bovine Norovirus is a detection assay for rapid and sensitive detection of RNA of bovine norovirus by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Bovine Norovirus

Product ViroReal® Bovine Norovirus
Order no. RTGV21
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene ORF1, detection in FAM channel
Content Assay (pimers + probe) for bovine norovirus
Positive Control bovine norovirus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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