ViroReal® Bovine Parainfluenzavirus 3


ViroReal Bovine Parainfluenza Virus 3



Background: Bovine parainfluenza virus 3 is an enveloped, single-stranded, negative-sense RNA ((-)ssRNA) virus, belonging to the Paramyxoviridae family and genetically and antigenically related to human parainfluenza virus 3. Three genotypes, A (BPIV3a), B (BPIV3b) and C (BPIV3c) have been described, based on genetic and phylogenetic analysis. BPIV3 infections are found in a wide variety of mammals including cattle, humans, sheep, goats, bisons, guinea pigs, rhinoceros, moose, bighorn sheep and camels. Cross-species infections have been reported. Usually, viral infection is asymptomatic or associated with only mild respiratory symptoms. In cattle it is associated with the clinical picture known as shipping fever. It is an important virus of the bovine respiratory disease complex. When coinfection with other pneumonia-associated agents (e.g. respiratory syncytial virus, bacteria) is present, severe respiratory disease may develop.

ViroReal® Bovine Parainfluenza Virus 3 is a detection assay for rapid and sensitive detection of RNA of the bovine parainfluenza virus 3 (BPIV3) genotype A, B and C by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Bovine Parainfluenza Virus 3

Product ViroReal® Bovine Parainfluenza Virus 3
Order no. RTGV24
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene Phosphoprotein P, detection in FAM channel
Content Assay (pimers + probe) for BPIV3
Positive Control BPIV3
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.

ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.