ViroReal® Bovine Torovirus


ViroReal Bovine TorovirusViroReal Assay FAM VET RNA mit Gefahrensymbol



Background: The species bovine torovirus (BToV) is a member of the family Coronaviridae, subfamily Torovirinae. The genome is a single-stranded, positive-sense RNA molecule. The kidney-shaped BToV causes diarrhea in calves but respiratory infections have also been reported and it has a worldwide distribution. Bovine torovirus, formerly called breda virus (BRV), was originally isolated from diarrheic calves in Breda, Iowa, in 1979.

ViroReal® Bovine Torovirus is a detection assay for rapid and sensitive detection of RNA of the bovine torovirus by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Bovine Torovirus 

Product ViroReal® Bovine Torovirus
Order no. RTGV90
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene M gene, detection in FAM channel
Content Assay (primers + probe) for bovine torovirus
Positive Control bovine torovirus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.

ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.