ViroReal® BRSV

 

ViroReal BRSV

 

 

Background: Bovine respiratory syncytial virus (BRSV) is an enveloped, non-segmented, single-stranded negative sense ((-)ssRNA) RNA virus that causes respiratory tract infections in cattle. It is an important virus in the bovine respiratory disease complex, affecting mostly young beef and dairy cattle. In stressful periods such as transportation or if co-infections are present (which is often the case in RSV infections), severe forms of pneumonia may develop. Treatment of co-infections with antibiotics, as well as disease control measurements are indicated.

ViroReal® BRSV is a detection assay for rapid and sensitive detection of RNA of the bovine respiratory syncytial virus (BRSV) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit BRSV

Product ViroReal® BRSV
Order no. RTGV25
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene Nucleoprotein (N), detection in FAM channel
Content Assay (pimers + probe) for BRSV
Positive Control BRSV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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