ViroReal® PCMV

 

ViroReal PCMV

 

Background: Porcine cytomegalovirus (PCMV) is a herpesvirus infecting only pigs. PCMV is present worldwide. Most PCMV infections are sub-clinical and  clinical disease is rare causing inclusion body rhinitis (IBR), pneumonia, anaemia, fever and abortion or neonatal piglet losses. The virus may alsobe endemic without causing any apparent clinical disease or economic loss. However, these infected animals can be latent carriers of the virus.

ViroReal® PCMV is a detection assay for rapid and sensitive detection of DNA of the porcine cytomegalovirus (PCMV) by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit PCMV

Product ViroReal® PCMV
Order no. RTGV40
Unit 100 reactions
Technology Real-time PCR
Target gene DPOL gene, detection in FAM channel
Content Assay (pimers + probe) for PCMV
Positive Control PCMV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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