ViroReal® PCV2


ViroReal PCV2



Background: The porcine circovirus is a non-enveloped virus with single-stranded and circular DNA. It can be classified into two types (PCV1 and PCV2) according to its antigenicity, pathogenicity and genomic difference. PCV1 is considered non-pathogenic while PCV2 infects pigs. PCV2-infection is widespread and essentially all pig herds are infected with PCV2 but relatively few have PCV2-associated disease (PCVAD) including postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS), and porcine proliferative and necrotizing pneumonia (PNP).

ViroReal® PCV2 is a detection assay for rapid and sensitive detection of DNA of the porcine circovirus type 2 (PCV2) including the so far three known genotypes (genotypes A, B and C) by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit PCV2

Product ViroReal® PCV2
Order no. RTGV100
Unit 100 reactions
Technology Real-time PCR
Target gene ORF1, detection in FAM channel
Content Assay (pimers + probe) for PCV2
Positive Control PCV2
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.