ViroReal® PEDV

 

ViroReal PEDVViroReal Assay FAM VET RNA mit Gefahrensymbol

 

 

Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. If endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. The PED virus is similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).

ViroReal® PEDV is a detection assay for rapid and sensitive detection of RNA of the porcine epidemic diarrhea virus (PEDV) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit PEDV

Product ViroReal® PEDV
Order no. RTGV80
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene N gene, detection in FAM channel
Content Assay (pimers + probe) for PEDV
Positive Control PEDV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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