ViroReal® PEDV & SDCV

 

ViroReal PEDV_SDCVViroReal Assay VET VIC und FAM 2 Posko RNA mit Gefahrensymbol

 

 

Background: The family Coronaviridae includes pathogenic porcine coronaviruses such as the transmissible gastroenteritis virus (TGEV), the porcine respiratory coronavirus (PRCV), the porcine epidemic diarrhea virus (PEDV), the swine delta coronavirus (SDCV, also called PDCoV) and the porcine hemmaglutinating encephalomyelitis virus (PHEV). TGEV causes transmissible gastroenteritis (diarrhea and vomiting) in pigs, mortality is highest in neonates. PRCV is a mutant of TGEV and does not appear to be an important primary pathogen. PEDV is similar to, but antigenically distinct from TGEV. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion of all ages of swine. If PEDV is endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. SDCV is a new virus first found in pigs in Hong Kong in 2012 and 2014 in the United States. SDCV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. SDCV infection is clinically similar to, but genetically distinct from porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV).

ViroReal® PEDV & SDCV is a detection assay for rapid and sensitive detection of RNA of SDCV (swine delta coronavirus, also called PDCoV),  as well as of PEDV (porcine epidemic diarrhea virus) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit PEDV & SDCV

Product ViroReal® PEDV & SDCV
Order no. RTGV69
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene 3´UTR region of SDCV and N gene of PEDV, detection in VIC/HEX and FAM channel
Content Assay (primers + VIC probe) for SDCV
Assay (primers + FAM probe) for PEDV
Positive Control SDCV
Positive Control PEDV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assay 3 (order no. RTGMIPCR3) contains primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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