ViroReal® PHEV

 

ViroReal PHEVViroReal Assay FAM VET RNA mit Gefahrensymbol

 

 

Background: Porcine hemagglutinating encephalomyelitis virus (PHEV), an RNA virus, is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae, and primarily infects pigs less than three weeks old. This viral disease of piglets is characterized by vomiting, anorexia and neurological symptoms and results either in rapid death or chronic emaciation (vomiting and wasting). The mortality rate in infected piglets under three weeks old is nearly 100 %, but the incidence of infection is small as most piglets are protected by maternal antibodies. Clinically apparent disease develops only when young piglets from non-immune sows are exposed. The virus is spread via aerosol.

ViroReal® PHEV is a detection assay for rapid and sensitive detection of RNA of the porcine hemagglutinating encephalomyelitis virus (PHEV) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit PHEV

Product ViroReal® PHEV
Order no. RTGV88
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene N gene, detection in FAM channel
Content Assay (pimers + probe) for PHEV
Positive Control PHEV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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