ViroReal® PRRS Virus EU & NA

 

ViroReal PRRS Virus EU NA

 

 

Background: The porcine reproductive and respiratory syndrome virus (PRRSV) is a single strain, positive-sense RNA virus belonging to the order Nidovirales, family Arteriviridae. This virus causes the Porcine Reproductive and Respiratory Syndrome (PRRS). PRRS is one of the most economically important diseases of swines and is also known as Blue-Ear Pig Disease. The disease is characterised by reproduction defects and by multifactor respiratory problems. European (EU) lineage PRRSV isolates (type 1, prototype strain is the Lelystad Virus) are genetically divergent from North American (US) isolates (type 2, prototype strain is the VR-2332) but cause similar clinical symptoms. A great genetic heterogeneity of field isolates is observed and further complicates diagnosis.

ViroReal® PRRS Virus EU & NA is a detection assay for rapid and sensitive detection of RNA of both viral lineages PRRSV EU and PRRSV NA NA (PRRSV type 1 and PRRSV type 2) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit PRRS Virus EU & NA

Product ViroReal® PRRS Virus EU & NA
Order no. RTGV1R01
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene ORF 7 and 3┬┤-untranslated region. Detection in FAM and VIC/HEX channel
Content Assay (pimers + probe) for PRRSV EU+ NA
Positive Control PRRSV EU+NA
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assay 3┬á(order no. RTGMIPCR3) contains primers, a probe and an internal control RNA target in an extra tube. See product description.

 ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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