ViroReal® Pseudorabiesvirus (SHV-1)

 

ViroReal PCV2

 

 

Background: Pseudorabiesvirus (PRV) is a swine DNA herpesvirus of the Alphaherpesvirinae subfamily. It is also known as suid herpesvirus 1 (SHV-1), or by its original name, Aujeszky’s disease virus. The pig is the principal reservoir host, but the virus may also infect other mammals like cattle, little ruminants, carnivores and rodents. It is not pathogenic for humans. In swine, AujeszkyÔÇÖs disease shows itself under three forms: a nervous form, a respiratory form or a genital form. In latently infected pigs, pseudorabiesvirus persists primarily in the trigeminal or sacral ganglia and the tonsils. In other mammals, it causes infection of the central nervous system with neurologic behavioral changes leading very quickly to death.

ViroReal┬«┬áPseudorabiesvirus (SHV-1) is a detection assay for rapid and sensitive detection of DNA of the pseudorabiesvirus (SHV-1,┬á Aujeszky’s disease virus) by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Pseudorabiesvirus (SHV-1)

Product ViroReal® Pseudorabiesvirus (SHV-1)
Order no. RTGV27
Unit 100 reactions
Technology Real-time PCR
Target gene Glycoprotein B, detection in FAM channel
Content Assay (pimers + probe) for SHV-1
Positive Control SHV-1
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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