ViroReal® Rabies Virus

 

ViroReal Rabies VirusViroReal Assay FAM VET RNA mit Gefahrensymbol

 

 

Background: Rabies is caused by a neurotropic virus of the genus Lyssavirus of the family Rhabdoviridae, and is transmissible to all mammals. As it is transmissible to humans by inoculation or inhalation of infectious virus, all suspected infected material must be handled under the appropriate safety conditions specified by the World Health Organisation (WHO). Previous known lyssavirus genotypes were recently reclassified, and the Lyssavirus genus currently consists of 14 lyssavirus species (Aravan virus, Australian bat lyssavirus (ABLV), Bokeloh bat lyssavirus (BBLV), Duvenhage virus (DUVV), European bat lyssavirus 1 (EBLV-1), European bat lyssavirus 2 (EBLV-2), Ikoma lyssavirus (IKOV), Irkut virus, Khujand virus, Lagos bat virus (LBV), Mokola virus (MOKV), Rabies virus (RABV), Shimoni bat virus (SHIBV) and the West Caucasian bat virus (WCBV). All lyssaviruses cause the severe neurotropic disease known as rabies. RABV (genotype 1, RABV-GT-1) is detected worldwide and is the prototype lyssavirus, the remaining species are known as rabies-related lyssaviruses. Human infections are mainly caused by RABV, DUVV, EBLV-1, EBLV-2 and ABLV.

ViroReal® Rabies Virus is a detection assay for rapid and sensitive detection of RNA of the classical rabies virus (RABV genotype 1, serotype 1, RABV-GT-1) by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Rabies Virus

Product ViroReal® Rabies Virus
Order no. RTGV255
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene Nucleoprotein (N), detection in FAM channel
Content Assay (pimers + probe) for RABV
Positive Control RABV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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