ViroReal® Rotavirus

 

ViroReal Rotavirus_RTGV

 

 

Background: Rotavirus is a segmented double-stranded (ds) RNA virus of the family Reoviridae and is classified into at least 7 groups (A to G). This virus is the causative agent of severe diarrhoea in almost every mammal species (especially cattle, pig, dog, cat horse and humans) and birds. Rotaviruses are thought to be mostly species specific, but some transmission between species occurs. Rotaviruses of group A–C infect both humans and animals, while rotaviruses of group D–G infect only animals. Group A is the most prevalent one and causes approximately 90% of infections in animals and humans. Furthermore, pigs can be sporadically also infected by group B, C and E. Cattles are sporadically also infected by group B and C. Group D, F and G are found in avians.

ViroReal® Rotavirus is a detection assay for rapid and sensitive detection of RNA of rotavirus genotype A, of most strains of genotype B and C, and of some strains of the other genotypes D, E, F and G by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit Rotavirus

Product ViroReal® Rotavirus
Order no. RTGV70
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene NSP4 gene, detection in FAM channel
Content Assay (pimers + probe) for rotavirus
Positive Control rotavirus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assays (order no. RTGMIPCR1, 2 or 3) contain primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

preloaderpreloaderpreloaderpreloader