ViroReal® TGEV

 

ViroReal TGEVViroReal Assay VIC VET RNA mit Gefahrensymbol

 

 

Background: The family Coronaviridae includes pathogenic porcine coronaviruses such as the transmissible gastroenteritis virus (TGEV), the porcine respiratory coronavirus (PRCV), the porcine epidemic diarrhea virus (PEDV) and the porcine hemmaglutinating encephalomyelitis virus (PHEV). TGEV causes transmissible gastroenteritis (diarrhea and vomiting) in pigs, mortality is highest in neonates. PRCV is a mutant of TGEV and does not appear to be an important primary pathogen.

ViroReal® TGEV is a detection assay for rapid and sensitive detection of RNA of the transmissible gastroenteritis virus (TGEV)  by reverse transcription real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: ViroReal® Kit TGEV

Product ViroReal® TGEV
Order no. RTGV60
Unit 100 reactions
Technology Reverse transcription real-time PCR
Target gene S region, detection in VIC/HEX channel
Content Assay (primers + probe) for TGEV
Positive Control TGEV
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For control of RNA extraction and exclusion of false-negative interpretation of results caused by inhibition of reverse transcription and real-time PCR an ingenetix Internal Positive RNA Control Assay can be optionally performed in a multiplex PCR, depending on the PCR-platform. Internal Positive RNA Control Assay 3┬á(order no. RTGMIPCR3) contains primers, a probe and an internal control RNA target in an extra tube. See product description.


ViroReal® Assays detecting viral RNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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