ViroReal® Kit Bovine Nebovirus

 

ViroReal KIT Bovine NebovirusViroReal Kit FAM_RNA mit Gefahren Symbol

 

 

ViroReal® Kit Bovine Nebovirus

Order no.

Reactions

Pathogen

Internal positive control

DVEV02713

100

FAM channel

Cy5 channel

DVEV02753

50

FAM channel

Cy5 channel

DVEV02711

100

FAM channel

VIC/HEX channel

DVEV02751

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for bovine nebovirus
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for bovine nebovirus

Background: Neboviruses are non-enveloped, single-stranded, linear, positive-sense RNA viruses ((+)ssRNA), belonging to the Caliciviridae family. The only currently known species in the genus Nebovirus is Newbury-1 virus. Cattle, especially calves, serve as natural hosts. Neboviral infection is associated with gastrointestinal disorders and necrotizing hepatitis leading to fatal hemorrhages in calves. The disease often remains endemic due to the lack of a commercially available vaccine.

Description: ViroReal® Kit Bovine Nebovirus is based on the amplification and detection of the ORF 1 of bovine nebovirus using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit Bovine Nebovirus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® Bovine Nebovirus


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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