ViroReal® Kit Bovine Torovirus 

 

ViroReal Kit Bovine TorovirusViroReal Kit FAM_RNA mit Gefahren Symbol

 

ViroReal® Kit Bovine Torovirus
Order no. Reactions Pathogen

Internal positive control

DVEV01613

100

FAM channel

Cy5 channel

DVEV01653

50

FAM channel

Cy5 channel

DVEV01611

100

FAM channel

VIC/HEX channel

DVEV01651

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for bovine torovirus
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for bovine torovirus

Background: The species bovine torovirus (BToV) is a member of the family Coronaviridae, subfamily Torovirinae. The genome is a single-stranded, positive-sense RNA molecule. The kidney-shaped BToV causes diarrhea in calves but respiratory infections have also been reported and it has a worldwide distribution. Bovine torovirus, formerly called breda virus (BRV), was originally isolated from diarrheic calves in Breda, Iowa, in 1979.

Description: ViroReal® Kit Bovine Torovirus is based on the amplification and detection of the membrane protein (M) gene of the BToV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit Bovine Torovirus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® Bovine Torovirus


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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