ViroReal® Kit BVD Virus 

 

ViroReal Kit BVD VirusBVDV Kit

 

 

Approved by Friedrich-Loeffler-Institute (Nr. FLI-B661)

ViroReal® Kit BVD Virus
Order no. Reactions Pathogen

Internal positive control

DVEV00613

100

FAM channel

Cy5 channel

DVEV00653

50

FAM channel

Cy5 channel

 

Kit contents:
Detection assay for BVD virus
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for BVD virus

Background: The genus Pestivirus is composed of 4 important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus,” was identified. The BVD virus is a single strain, positive-sense RNA virus found throughout the world and causes bovine viral diarrhoea (BVD). BVD is a disease of cattle which causes various clinical syndromes including diarrhea, mucosal disease, reproductive dysfunctions and/or hemorrhagic syndrome. Prenatal infection by BVD results in cattles permanently infected (BVD-PI). Such animals carry the virus throughout their lifetime and constantly secrete large numbers of viral particles.

Description: ViroReal® Kit BVD Virus is based on the amplification and detection of the 5´UTR of the BVD virus genome using one-step reverse transcription real-time PCR. It also reacts with some strains of CSFV and BDV. It allows the rapid and sensitive detection of RNA of BVDV-1, BVDV-2 and BVDV-3.

PCR-platforms: ViroReal® Kit BVD Virus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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