ViroReal® Kit EMCV

 

ViroReal Kit EMCVViroReal Kit FAM_RNA mit Gefahren Symbol

 

ViroReal® Kit EMCV
Order no. Reactions Pathogen

Internal positive control

DVEV01013

100

FAM channel

Cy5 channel

DVEV01053

50

FAM channel

Cy5 channel

DVEV01011

100

FAM channel

VIC/HEX channel

DVEV01051

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for encephalomyocarditis virus (EMCV)
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for EMCV

Background: Encephalomyocarditis virus (EMCV) is one of three virus species in the genus Cardiovirus in the family Picornaviridae and is a single stranded RNA (ssRNA) virus that causes encephalomyocarditis and reproductive disease in swine and zoologic mammals. Infected piglets suffer from encephalitis, myocarditis and sudden death. Reproductive problems include poor conception rates, embryo resorption, mummification, stillbirths, abortions and neonatal death. The disease affects the nervous, reproductive, respiratory and circulatory system of pigs. Fatal myocarditis due to EMC virus infection has been seen in primates, even-and odd-toed ungulates, elephants, carnivores and rodents. The disease can be found worldwide. The most important mode of virus transmission seems to be rodent-to-pig transmission, and rodents act as the major reservoir of the disease. Rats and other rodents probably infect pigs directly or through diseased carcasses and contaminated feeds or water supplies.

Description: ViroReal® Kit EMCV is based on the amplification and detection of the 3´UTR RNA of EMCV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit EMCV is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® EMCV


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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