ViroReal® Kit PCV2

 

ViroReal Kit PCV2

 

ViroReal® Kit PCV2
Order no. Reactions Pathogen

Internal positive control

DVEV00513

100

FAM channel

Cy5 channel

DVEV00553

50

FAM channel

Cy5 channel

DVEV00511

100

FAM channel

VIC/HEX channel

DVEV00551

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for for PCV2
Detection assay for internal positive control (control of amplification)
DNA reaction mix (including uracil-N glycosylase, UNG)
Positive control for PCV2
Water

Background: The porcine circovirus is a non-enveloped virus with single-stranded and circular DNA. It can be classified into two types (PCV1 and PCV2) according to its antigenicity, pathogenicity and genomic difference. PCV1 is considered non-pathogenic while PCV2 infects pigs. PCV2-infection is widespread and essentially all pig herds are infected with PCV2 but relatively few have PCV2-associated disease (PCVAD) including postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS), and porcine proliferative and necrotizing pneumonia (PNP). From piglets showing PCVAD signs PCV2 can be isolated at high titres.

Description: ViroReal® Kit PCV2 is based on the amplification and detection of the ORF1 of the porcine circovirus type 2 (PCV2) including the three genotypes A, B and C known so far. The assay is designed to detect very small amounts of PCV2 but does not detect PCV1 or PPV. The assay allows determination of PCV2 genomic copy numbers in sample material.  Pigs with 107 PCV2 genomic copies/ml serum or greater are likely to have PCV2-associated lymphoid lesions and disease.

PCR-platforms: ViroReal® Kit PCV2 is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

A Quantitative real-time PCR Standard PCV2  Download: Product Description PC100 can be ordered separately (order no. PC100).

Also available as assay only: ViroReal® PCV2


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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