ViroReal® Kit PEDV

 

ViroReal Kit PEDVViroReal Kit FAM_RNA mit Gefahren Symbol

 

ViroReal®  Kit PEDV

Order no.

Reactions

Pathogen

Internal positive control

DVEV01313

100

FAM channel

Cy5 channel

DVEV01353

50

FAM channel

Cy5 channel

 

Kit contents:
Detection assay for porcine epidemic diarrhea virus (PEDV)
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for PEDV

Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. If endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. The PED virus is similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).

Description: ViroReal® Kit PEDV is based on the amplification and detection of the nucleocapsid protein gene (N gene) of PEDV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit PEDV is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® PEDV


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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