ViroReal® Kit PEDV & SDCV

 

ViroReal Kit PEDV_SDCVViroReal Kit VET_FAM VIC_RNA 2 Posko mit Gefahren Symbol

 

ViroReal® Kit PEDV & SDCV

Order no.

Reactions

Pathogen

Internal positive control

DVEV01913

100

FAM + VIC/HEX channel

Cy5 channel

DVEV01953

50

FAM + VIC/HEX channel

Cy5 channel

 

Kit contents:
– Detection assay for porcine epidemic diarrhea virus (PEDV)
– Detection assay for swine delta coronavirus (SDCV)
– Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
– RNA reaction mix
– Nuclease-free water
– Positive controls (RNA) for PEDV and SDCV

Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. If endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. The PED virus is similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV). Swine delta coronavirus (SDCV), also called porcine delta coronavirus (PDCoV), is a member of the genus Coronavirus (Deltacoronavirus), family Coronaviridae and a positive-sense, enveloped, single-stranded RNA virus. SDCV is a new virus first found in pigs in Hong Kong in 2012 and 2014 in the United States. SDCV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. SDCV infection is clinically similar to, but genetically distinct from porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV).

Description: ViroReal┬« Kit PEDV & SDCV is based on the amplification and detection of the nucleocapsid protein gene (N gene) of PEDV and the 3ÔÇÖUTR of SDCV using one-step reverse transcription real-time PCR. The PED virus is detected in the FAM channel. The SDC virus is detected in the VIC/HEX channel.

PCR-platforms: ViroReal® Kit PEDV & SDCV is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® PEDV & SDCV

 


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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