ViroReal® Kit PEDV & TGEV

 

ViroReal Kit PEDV_TGEVViroReal Kit VET_FAM VIC_RNA 2 Posko mit Gefahren Symbol

 

ViroReal® Kit PEDV & TGEV

Order no.

Reactions

Pathogen

Internal positive control

DVEV01413

100

FAM + VIC/HEX channel

Cy5 channel

DVEV01453

50

FAM + VIC/HEX channel

Cy5 channel

 

Kit contents:
– Detection assay for porcine coronavirus PEDV
– Detection assay for porcine coronavirus TGEV
– Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
– RNA reaction mix
– Nuclease-free water
– Positive controls (RNA) for PEDV and TGEV

Background: The family Coronaviridae includes pathogenic porcine coronaviruses such as the transmissible gastroenteritis virus (TGEV), the porcine respiratory coronavirus (PRCV), the porcine epidemic diarrhea virus (PEDV) and the porcine hemmaglutinating encephalomyelitis virus (PHEV). TGEV causes transmissible gastroenteritis (diarrhea and vomiting) in pigs, mortality is highest in neonates. PRCV is a mutant of TGEV and does not appear to be an important primary pathogen. PEDV is similar to, but antigenically distinct from TGEV. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion of all ages of swine. If PEDV is endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs.

Description: ViroReal┬« Kit PEDV & TGEV is based on the amplification and detection of the nucleocapsid protein gene (N gene) of PEDV as well as a part of the S region of TGEV using one-step reverse transcription real-time PCR. PEDV is detected in FAM channel. TGEV is detected in VIC/HEX channel. The test clearly differentiates between these two viruses and does not detect PRCV with a large in-frame deletion in the 5ÔÇÖ end of the S gene.

PCR-platforms: ViroReal® Kit PEDV & TGEV is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® PEDV & TGEV

 


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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