ViroReal® Kit PHEV

 

ViroReal Kit PHEVViroReal Kit FAM_RNA mit Gefahren Symbol

 

ViroReal® Kit PHEV
Order no. Reactions Pathogen

Internal positive control

DVEV01513

100

FAM channel

Cy5 channel

DVEV01553

50

FAM channel

Cy5 channel

DVEV01511

100

FAM channel

VIC/HEX channel

DVEV01551

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for porcine hemagglutinating encephalomyelitis virus (PHEV)
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for PHEV

Background: Porcine hemagglutinating encephalomyelitis virus (PHEV), an RNA virus, is a member of the genus Coronavirus (Betacoronavirus), family Coronaviridae, and primarily infects pigs less than three weeks old. This viral disease of piglets is characterized by vomiting, anorexia and neurological symptoms and results either in rapid death or chronic emaciation (vomiting and wasting). The mortality rate in infected piglets under three weeks old is nearly 100 %, but the incidence of infection is small as most piglets are protected by maternal antibodies. Clinically apparent disease develops only when young piglets from non-immune sows are exposed. The virus is spread via aerosol.

Description: ViroReal® Kit PHEV is based on the amplification and detection of the nucleocapsid protein gene (N gene) of the PHEV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit PHEV is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® PHEV


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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