ViroReal® Kit PRRS Virus EU & NA

 

ViroReal Kit PRRS Virus EU NA

 

 

ViroReal® Kit PRRS Virus EU & NA

Order no.

Reactions

Pathogen

Internal positive control

DVEV00113

100

FAM + VIC/HEX channel

Cy5 channel

DVEV00153

50

FAM + VIC/HEX channel

Cy5 channel

 

Kit contents:
Detection assay for PRRS virus EU and NA
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
– RNA reaction mix
Nuclease-free water
Positive control (RNA) for PRRS virus EU and NA

Background: The porcine reproductive and respiratory syndrome virus (PRRSV) is a single strain, positive-sense RNA virus belonging to the order Nidovirales, family Arteriviridae. This virus causes the Porcine Reproductive and Respiratory Syndrome (PRRS). PRRS is one of the most economically important diseases of swines and is also known as Blue-Ear Pig Disease. The disease is characterised by reproduction defects and by multifactor respiratory problems. European (EU) lineage PRRSV isolates (type 1, prototype strain is the Lelystad Virus) are genetically divergent from North American (US) isolates (type 2, prototype strain is the VR-2332) but cause similar clinical symptoms. A great genetic heterogeneity of field isolates is observed and further complicates diagnosis..

Description: ViroReal┬« Kit PRRS Virus EU & NA is based on the amplification and detection of the open-reading frame 7 including the 3′-untranslated region of both viral lineages PRRS virus EU and PRRS virus NA (PRRSV type 1 and PRRSV type 2)┬áusing one-step reverse transcription real-time PCR. The PRRS virus EU lineage is detected in FAM channel. The PRRS virus NA lineage is detected in VIC/HEX channel.

PCR-platforms: ViroReal® Kit PRRS Virus EU & NA is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® PRRS Virus EU & NA


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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