ViroReal® Kit Pseudorabiesvirus (SHV-1)

 

ViroReal KIT PseudorabiesViroReal Kit FAM_RNA mit Gefahren Symbol

 

 

ViroReal® Kit Pseudorabiesvirus (SHV-1)

Order no.

Reactions

Pathogen

Internal positive control

DVEV02513

100

FAM channel

Cy5 channel

DVEV02553

50

FAM channel

Cy5 channel

DVEV02511

100

FAM channel

VIC/HEX channel

DVEV02551

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for┬ápseudorabiesvirus

– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for┬ápseudorabiesvirus
– Water

Background: Pseudorabiesvirus (PRV) is a swine DNA herpesvirus of the Alphaherpesvirinae subfamily. It is also known as suid herpesvirus 1 (SHV-1), or by its original name, Aujeszky’s disease virus. The pig is the principal reservoir host, but the virus may also infect other mammals like cattle, little ruminants, carnivores and rodents. It is not pathogenic for humans. In swine, AujeszkyÔÇÖs disease shows itself under three forms: a nervous form, a respiratory form or a genital form. In latently infected pigs, pseudorabiesvirus persists primarily in the trigeminal or sacral ganglia and the tonsils. In other mammals, it causes infection of the central nervous system with neurologic behavioral changes leading very quickly to death.

Description: ViroReal® Kit Pseudorabiesvirus (SHV-1) is based on the amplification and detection of the glycoprotein B gene of pseudorabiesvirus (also called PRV, SHV-1) using real-time PCR.

PCR-platforms: ViroReal® Kit Pseudorabiesvirus (SHV-1) is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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