ViroReal® Kit Rabies Virus

 

ViroReal Kit Rabies VirusViroReal Kit FAM_RNA mit Gefahren Symbol

 

 

ViroReal® Kit Rabies Virus

Order no.

Reactions

Pathogen

Internal positive control

DVEV00813

100

FAM channel

Cy5 channel

DVEV00853

50

FAM channel

Cy5 channel

DVEV00811

100

FAM channel

VIC/HEX channel

DVEV00851

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for rabies virus (RABV)
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for RABV

Background: Rabies is caused by a neurotropic virus of the genus Lyssavirus of the family Rhabdoviridae, and is transmissible to all mammals. As it is transmissible to humans by inoculation or inhalation of infectious virus, all suspected infected material must be handled under the appropriate safety conditions specified by the World Health Organisation (WHO). Previous known lyssavirus genotypes were recently reclassified, and the Lyssavirus genus currently consists of 14 lyssavirus species (Aravan virus, Australian bat lyssavirus (ABLV), Bokeloh bat lyssavirus (BBLV), Duvenhage virus (DUVV), European bat lyssavirus 1 (EBLV-1), European bat lyssavirus 2 (EBLV-2), Ikoma lyssavirus (IKOV), Irkut virus, Khujand virus, Lagos bat virus (LBV), Mokola virus (MOKV), Rabies virus (RABV), Shimoni bat virus (SHIBV) and the West Caucasian bat virus (WCBV). All lyssaviruses cause the severe neurotropic disease known as rabies. RABV (genotype 1, RABV-GT-1) is detected worldwide and is the prototype lyssavirus, the remaining species are known as rabies-related lyssaviruses. Human infections are mainly caused by RABV, DUVV, EBLV-1, EBLV-2 and ABLV.

Description: ViroReal® Kit Rabies Virus is based on the amplification and detection of the nucleoprotein (N) gene of RABV using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit Rabies Virus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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