ViroReal® Kit Rotavirus

 

ViroReal Kit RotavirusViroReal Kit FAM_RNA mit Gefahren Symbol

 

ViroReal® Kit Rotavirus
Order no. Reactions Pathogen

Internal positive control

DVEV01713

100

FAM channel

Cy5 channel

DVEV01753

50

FAM channel

Cy5 channel

DVEV01711

100

FAM channel

VIC/HEX channel

DVEV01751

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for rotavirus
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for rotavirus

Background:┬áRotavirus is a segmented double-stranded (ds) RNA virus of the family Reoviridae and is classified into at least 7 groups (A to G). This virus is the causative agent of severe diarrhoea in almost every mammal species (especially cattle, pig, dog, cat horse and humans) and birds. Rotaviruses are thought to be mostly species specific, but some transmission between species occurs. Rotaviruses of group AÔÇôC infect both humans and animals, while rotaviruses of group DÔÇôG infect only animals. Group A is the most prevalent one and causes approximately 90% of infections in animals and humans. Furthermore, pigs can be sporadically also infected by group B, C and E. Cattles are sporadically also infected by group B and C. Group D, F and G are found in avians.

Description: ViroReal® Kit Rotavirus is based on the amplification and detection of a part of the non-structural protein 4 (NSP4), a transmembrane ER-specific glycoprotein, of rotavirus genotype A, of most strains of genotype B and C, and of some strains of the other genotypes D, E, F and G using one-step reverse transcription real-time PCR.

PCR-platforms: ViroReal® Kit Rotavirus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity: See product description

Also available as assay only: ViroReal® Rotavirus


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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