ViroReal® Kit TGEV

 

ViroReal Kit TGEVViroReal Kit VIC_RNA mit Gefahren Symbol

 

ViroReal®  Kit TGEV

Order no.

Reactions

Pathogen

Internal positive control

DVEV01213

100

VIC/HEX channel

Cy5 channel

DVEV01253

50

VIC/HEX channel

Cy5 channel

 

Kit contents:
Detection assay for TGEV
Detection assay+target for internal RNA positive control (control of RT-PCR/RNA extraction)
RNA reaction mix
Nuclease-free water
Positive control (RNA) for TGEV

Background: The family Coronaviridae includes pathogenic porcine coronaviruses such as the transmissible gastroenteritis virus (TGEV), the porcine respiratory coronavirus (PRCV), the porcine epidemic diarrhea virus (PEDV) and the porcine hemmaglutinating encephalomyelitis virus (PHEV). TGEV causes transmissible gastroenteritis (diarrhea and vomiting) in pigs, mortality is highest in neonates. PRCV is a mutant of TGEV and does not appear to be an important primary pathogen.

Description: ViroReal® Kit TGEV is based on the amplification and detection of a part of the S region of TGEV using one-step reverse transcription real-time PCR. The test does not detect PRCV.

PCR-platforms: ViroReal® Kit TGEV is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Specificity and sensitivity: See product description

Also available as assay only: ViroReal® TGEV


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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