Background: Acinetobacter baumannii is a Gram-negative, aerobic bacterium which is responsible for nosocomial infections. It occurs mainly in intensive care units. This bacterium is often resistant to antibiotics and thus difficult to treat. It can lead to pneumonia, catheter-associated infections and can also cause sepsis. Proteus mirabilis is a Gram-negative, facultatively anaerobic rod-shaped bacterium which frequently occurs in the large intestine and is generally non-pathogenic. However, in people with a weakened immune system, it can lead to urinary tract infections, wound infections, pneumonia and sepsis.

Description: BactoReal® Kit A. baumannii & P. mirabilis is a non-automatic in-vitro diagnostic real-time PCR test for the qualitative detection and identification of extracted DNA of Acinetobacter baumannii (rpoB gene) and Proteus mirabilis (ureC gene) from samples purified from human EDTA blood, aspirates and CSF.  A probe-specific amplification curve in the fluorescence channel for FAM or VIC detects the pathogen-specific DNA. The Internal Positive Control (IPC) is detected in the fluorescence channel Cy5 and serves as a control for DNA extraction and possible real-time PCR inhibition. The target for the DNA IPC is added during sample extraction.In combination with other diagnostic tests such as culture it supports a rapid and specific diagnosis for patients suspected of having a bacterial infection. Results have to be interpreted in context of the overall picture and other clinical parameters. Sepsis diagnosis must not be based solely on BactoReal® Kit A. baumannii & P. mirabilis.

Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit A. baumannii & P. mirabilis
OrdernumberReactionsChannel PathogenChannel IPCTarget
DHUS0065350FAM, VICCY5rpoB + ureC
For in vitro diagnostic use.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: rpoB gene of Acinetobacter baumannii and ureC gene of Proteus mirabilis
  • CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Declaration of Conformity

PCR platforms: BactoReal® Kit A. baumannii & P. mirabilis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific) and cobas z 480 Analyzer (Roche), but is also suitable for other real-time PCR instruments.

BactoReal®, MycoReal, PanReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.