Background: One of the six eukaryotic clusters comprise the Amoebozoa, of which the genera Acanthamoeba is primarily a free-living protozoan in natural habitats, but also causative agent of human and animal disease. Acanthamoeba serves as host for a variety of pathogenic bacteria such as Mycobacterium avium. Infestation with Acanthamoeba is associated with potentially sight-threatening contact lens-related keratitis, serious infections of other organs and fatal granulomatous amoebic encephalitis. More than 20 species of Acanthamoeba are known, which can be classified into three morphologic groups (Group I, II and III) and 15 genotypes (T1-T15). Some species (A. astronyxis, A. byersi, A. castellanii, A. culbertsoni, A. divionensis, A. griffinii, A. hatchetti, A. healyii, A. lenticulata, A. lugdunensis, A. palestinensis, A. polyphaga, A. quina, A. rhysodes) have recently been associated with human disease. Genotype T4 has been considered the most important genotype in both ocular and CNS infections.

Description: ParoReal Kit Acanthamoeba uses real-time PCR for the detection of the 18S rRNA gene of Acanthamoeba. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination.For more detailed information see here.

ParoReal Kit Acanthamoeba
OrdernumberReactionsChannel PathogenChannel IPCTarget
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: 18S rRNA gene of Acanthamoeba
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal Kit Acanthamoeba is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal Acanthamoeba
OrdernumberReactionsChannel PathogenChannel IPCTarget
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.