Background: Aspergillus is a fungal genus consisting of several hundred species. Aspergillus species are ubiquitously found and some species can cause opportunistic infections such as allergic bronchopulmonary aspergillosis, pulmonary aspergilloma and invasive aspergillosis. Invasive aspergillosis is increasingly recognized as a primary cause of morbidity and mortality especially in immunocompromised patients. Aspergillus fumigatus is the major disease causing Aspergillus species. However, other species than A. fumigatus, in particular A. terreus and A. flavus but also A. niger, A. nidulans and A. ustus have gained greater clinical significance.
Description: MycoReal Aspergillus is a real-time PCR detection assay for rapid and sensitive detection as well as differentiation of 5 medically important species of Aspergillus (A. fumigatus, A. flavus, A. nidulans, A. niger and A. terreus). Species can be differentiated by specific melting curves. By using species-specific probes the test is highly specific for Aspergillus. The identification of the species allows a first conclusion on a possible antimycotica resistance behaviour. Since MycoReal Assays (MycoReal Aspergillus, MycoReal Candida, MycoReal Pneumocystis and MycoReal Fungi) use identical temperature/time profiles, they can be combined in the same LightCycler® run. This allows the detection of a wide panel of pathogenic fungi within only a few hours.
- Amplification and detection: ITS2-Region of Aspergillus
- Real-time PCR with primers, probes and positive control
- Internal Positive Control System to exclude false-negative results
PCR platforms: MycoReal Aspergillus is developed and validated for the LightCycler® 2.0 instrument (Roche).
Assay: This product is available as an assay-only format with primers, probe and a positive control, but without reaction mix.
|Ordernumber||Reactions||Channel Pathogen||Channel IPC||Target|
|RTPM100||50||640 nm /705 nm||610 nm||ITS2-region|
Shipping Temperature: +4°C cool packs