Background: Bordetella bronchiseptica is a Gramnegative bacterium found as a normal inhabitant of the respiratory tract in a variety of animals such as dogs, pigs, some rodents, as well as humans. While it likely causes pulmonary infections in animals, it rarely provokes human disease. However, in immunocompromised humans (HIV-infection, organ transplantation), it may cause severe cases of pneumonia which require antibiotic treatment.

Description: BactoReal® Kit Bordetella bronchiseptica uses real-time PCR for the detection of bfrA gene of Bordetella bronchiseptica. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Bordetella bronchiseptica
OrdernumberReactionsChannel PathogenChannel IPCTarget
DHUB0245350FAMCY5bfrA gene
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: bfrA gene of Bordetella bronchiseptica
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Bordetella bronchiseptica is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bordetella bronchiseptica
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGM145050FAM-bfrA gene
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.