Background: Bordetella parapertussis is a Gramnegative bacterium of the genus Bordetella. Transmission occurs through close contact with contaminated respiratory droplets. While B. pertussis is the etiologic agent of whooping cough, B. parapertussis, B. holmesii and B. bronchiseptica may cause a pertussis-like respiratory disease, but with a milder clinical course. B. pertussis and B. parapertussis are exclusively human pathogens, whereas B. bronchiseptica most often infects animals, but is infrequently isolated also from humans. A frequently used target for PCR detection of B. parapertussis is the insertion sequence IS1001, which is absent in B. pertussis but found in B. parapertussis as well as in some strains of B. holmesii and B. bronchiseptica.

Description: BactoReal® Kit Bordetella parapertussis uses real-time PCR for the detection of the insertion sequence IS1001 of Bordetella parapertussis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Bordetella parapertussis
OrdernumberReactionsChannel PathogenChannel IPCTarget
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: IS1001 of Bordetella parapertussis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Bordetella parapertussis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bordetella parapertussis
OrdernumberReactionsChannel PathogenChannel IPCTarget
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.