Background: Bordetella pertussis is a Gramnegative bacterium of the genus Bordetella and the etiologic agent of whooping cough, an acute respiratory disease. Transmission occurs through close contact with contaminated respiratory droplets. A pertussis-like respiratory disease, but with a milder clinical course may be caused by B. parapertussis, B. holmesii and B. bronchiseptica. While B. pertussis and B. parapertussis are exclusively human pathogens, B. bronchiseptica most often infects animals, but is infrequently isolated also from humans. A frequently used target for PCR detection of B. pertussis is the insertion sequence IS481, which is absent in B. parapertussis, but found in B. pertussis and B. holmesii as well as in some strains of B. bronchiseptica.

Description: BactoReal® Kit Bordetella pertussis uses real-time PCR for the detection of insertion sequence IS481 of Bordetella pertussis. Our carefully designed primers and probe ensure highest sensitivity and specificity. An additional analysis with BactoReal® Kit Bordetella parapertussis (ingenetix order no. DHUB00253) can largely differ between an infection with B. pertussis or B. holmesii / B. bronchiseptica. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Bordetella pertussis
OrdernumberReactionsChannel PathogenChannel IPCTarget
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: IS481 of Bordetella pertussis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Bordetella pertussis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bordetella pertussis
OrdernumberReactionsChannel PathogenChannel IPCTarget
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.