Background: Escherichia coli is a Gramnegative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors, which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. Enteropathogenic Escherichia coli (EPEC) produces an outer membrane protein called intimin, encoded by eae and mediating intestinal cell attachment. Intimin is a virulence factor (adhesin) of EPEC and EHEC strains. Furthermore, EPEC are classified into typical and atypical strains based on the presence of the plasmid E. coli adherence factor (EAF). Typical EPEC strains are rarely isolated from animals, and humans are the major natural reservoir. The reservoir for atypical EPEC can be both animals and humans. By definition all EPEC lack genes to produce shiga toxin (stx).
Description: BactoReal® E. coli Toxin Typing is a real-time PCR test for detection of virulence-associated genes of E. coli. It allows the detection of DNA of enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and of Shiga toxin (verotoxin) producing E. coli (STEC) purified from isolated bacterial colonies. Stx1 is detected in FAM channel. Stx2 is detected in VIC/HEX channel. Eae is detected in FAM channel. EhxA is detected in VIC/HEX channel. Assays for stx1 and stx2, as well as assays for eae and ehxA can be combined in a multiplex PCR.
- Amplification and detection: virulence-associated genes eae, ehxA, stx1, stx2 of E. coli
- Real-time PCR Assay with primers, probes and postitve controls for 50 reactions each
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® E. coli Toxin Typing is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
|Ordernumber||Reactions||Channel Pathogen||Channel IPC||Target|
|RTGM1280||50 x 4||FAM & VIC/HEX||-||eae, stx1 & ehxA, stx2|
Shipping Temperature: +4°C in cool packs