Background: Human herpes virus type 8 (HHV-8) is a double-stranded DNA virus. This virus is primarily transmitted through sexual contact, but also via blood and possibly also via saliva. HHV-8, also called Kaposi´s sarkoma-associated-herpesvirus (KSHV), is associated with Kaposi's sarcoma (KS), some malignant lymphomas and some forms of multicentric Castleman´s disease (MCD) mainly in human beings with immunodeficiency.

Description: ViroReal® Kit HHV-8 uses real-time PCR for the detection of the ORF 26 of HHV-8. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

ViroReal® Kit HHV-8
OrdernumberReactionsChannel PathogenChannel IPCTarget
DHUV0085350FAMCY5ORF 26
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: ORF 26 of HHV-8
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit HHV-8 is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® HHV-8
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGM18V50FAM-ORF 26
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.