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Download All Background: Listeria are ubiquitously found in soil and water. Listeriosis is an infection caused by the consumption of contaminated food (milk, cheeses, ice cream, vegetables, sausages, meats and fish). Although there are other types of Listeria (L. ivanovii, L. innocua, L. welshimeri, L. seegligeri and L. grayi), the culprit pathogen for most listeriosis cases is L. monocytogenes. Gastrointestinal symptoms may remain the only symptoms or precede more severe clinical manifestations such as sepsis and meningitis. People at risk include the elderly population, immunosuppressed patients and foetuses of pregnant women. L. monocytogenes is a Grampositive, facultative anaerobic, and facultative intracellular bacterium with an incubation time of at least seven days. It expresses a Beta hemolysin (hlyA gene) which causes destruction of red blood cells and has the ability to target other cells. The ability of L. monocytogenes to grow at temperatures as low as 0°C permits multiplication in refrigerated foods. L. monocytogenes is killed by pasteurization and cooking.
Description: BactoReal® Kit Listeria monocytogenes uses real-time PCR for the detection of the hlyA gene of Listeria monocytogenes. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUB00853 | 50 | FAM | Cy5 | hlyA gene |
- Amplification and detection: hlyA gene of Listeria monocytogenes
- CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Listeria monocytogenes is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.