Background: Human parechoviruses are non-enveloped, positive single-stranded RNA viruses (ss(+)RNA) and are members of the Picornaviridae family. Six serotypes of human parechovirus have been identified (human parechovirus 1-6). Parechoviruses are responsible for gastrointestinal, respiratory and central nervous system infections. 95% of humans are carriers of human parechovirus since early life.

Description: ViroReal® Kit Parechovirus uses RT real-time PCR for the detection of the polyprotein gene of human parechovirus strains 1-6. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit Parechovirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
DHUV0175350FAMCY5polyprotein gene
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: polyprotein gene of Parechovirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Parechovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Parechovirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGM36RV50FAM-polyprotein gene
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.