Background: Pneumocystis jiroveci (formerly Pneumocystis carinii) is a yeast-like fungus which can be found worldwide. Pneumocystis jiroveci is a distinct species that only infects humans, while the related species P. carinii can be found in rodents and other mammals. Airborne transmission of Pneumocystis from host to host has been demonstrated in rodent models and several observations suggest that interindividual transmission occurs in humans. Both healthy and immunocompromised people can be colonised with P. jiroveci. While it does not affect healthy people, P. jiroveci can cause an interstitial Pneumocystis-pneumonia (PCP) in HIV-patients, persons with primary immune deficiencies, including hypogammaglobulinemia and severe combined immunodeficiency (SCID), patients receiving long-term immunosuppressive regimens for connective-tissue disorders, vasculitides, or solid-organ transplantation, patients with hematologic and nonhematologic malignancies, including solid tumors and lymphomas, and persons with severe malnutrition. Currently the diagnosis of PCP relies on microscopic methods or PCR, as P. jiroveci cannot be cultured in routine microbiology laboratories.
Description: MycoReal Kit Pneumocystis uses real-time PCR for the detection of the mitochondrial large-subunit rRNA gene (mt LSU) of P. jiroveci. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.
|Ordernumber||Reactions||Channel Pathogen||Channel IPC||Target|
Shipping Temperature: +4°C cool packs
- Amplification and detection: mt LSU of Pneumocystis jiroveci
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: MycoReal Kit Pneumocystis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.