Background: Pneumocystis jiroveci (formerly Pneumocystis carinii) is a yeast-like fungus which can be found worldwide. Pneumocystis jiroveci is a distinct species that only infects humans, while the related species P. carinii can be found in rodents and other mammals. Airborne transmission of Pneumocystis from host to host has been demonstrated in rodent models and several observations suggest that interindividual transmission occurs in humans. Both healthy and immunocompromised people can be colonised with P. jiroveci. While it does not affect healthy people, P. jiroveci can cause an interstitial Pneumocystis-pneumonia (PCP) in persons with HIV infection, with primary immune deficiencies, including hypogammaglobulinemia and severe combined immunodeficiency (SCID), persons receiving long-term immunosuppressive regimens for connective-tissue disorders, vasculitides, or solid-organ transplantation, persons with hematologic and nonhematologic malignancies, including solid tumors and lymphomas, and persons with severe malnutrition. Currently the diagnosis of PCP relies on microscopic methods or PCR, as P. jiroveci cannot be cultured in routine microbiology laboratories.
Description: MycoReal Pneumocystis is based on the amplification and detection of the mitochondrial large-subunit rRNA gene (mt LSU) gene of P. jiroveci using real-time PCR. It allows the rapid and sensitive detection of the mt LSU gene of P. jiroveci from DNA samples purified from bronchoalveolare lavages (BAL), tissue and paraffin embedded tissue. Since MycoReal Assays (MycoReal Aspergillus, MycoReal Candida, MycoReal Pneumocystis and MycoReal Fungi) use identical temperature/time profiles, they can be combined in the same LightCycler® 1.2/1.5/2.0 run. This allows the detection of a wide panel of pathogenic fungi within only a few hours.
- Amplification and detection: mt LSU gene of P. jiroveci
- Real-time PCR with primern, probes and positive control
- Internal Positive Control System to exclude false-negative results
PCR platforms: MycoReal Pneumocystis is developed and validated for the LightCycler® 1.2/1.5/2.0 and 480 instruments (Roche), the ABI PRISM® 7500 (Fast) instrument (Applied Biosystems), and Mx3005P® QPCR System (Agilent) but is also suitable for other real-time PCR instruments. Order number for the respective PCR-platforms see below.
Assay: This product is available as an assay-only format with primers, probe and a positive control, but without reaction mix.
|Ordernumber||Reactions||Channel Pathogen||Channel IPC||Target|
|RTPM401||50||FAM||VIC/HEX||ABI PRISM® 7500 (Fast) Instruments / Mx3005P® QPCR|
|RTPM403||50||FAM||CY5||ABI PRISM® 7500 (Fast) Instruments / Mx3005P® QPCR / LightCycler® 480|
Shipping Temperature: +4°C cool packs