Background: Pseudomonas aeruginosa is a Gramnegative bacterium found widely in the environment, such as in soil, water, and plants. It usually does not cause infections in healthy people. If an infection occurs in a healthy person, it is generally mild. Serious Pseudomonas infections usually can be found in people in the hospital and/or with weakened immune systems. It is the most common pathogen isolated from hospitalized patients, and it is a frequent cause of nosocomial infections. Pseudomonal infections are complicated and can be life-threatening. In these people infections of the blood, pneumonia, and infections following surgery can lead to severe illness and death.

Description: BactoReal® Kit Pseudomonas aeruginosa uses real-time PCR for the detection of the 16S rRNA gene of Pseudomonas aeruginosa. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

BactoReal® Kit Pseudomonas aeruginosa
OrdernumberReactionsChannel PathogenChannel IPCTarget
DHUB0265350FAMCY516S rDNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: 16S rRNA gene of Pseudomonas aeruginosa
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Pseudomonas aeruginosa is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Pseudomonas aeruginosa
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGM90650FAM-16S rDNA
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs

Related Products: BactoReal®Pseudomonas aeruginosa Standard

BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.