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Background: Varicella zoster virus (VZV), also called human herpes virus type 3 (HHV-3) is a double-stranded DNA virus. The VZV is found worldwide (> 95% of persons are seropositive) and is transmitted by droplet infection. Primary infection with VZV causes chickenpox. After primary infection, VZV remains dormant in sensory nerve roots for life. Upon reactivation, the virus migrates down the sensory nerve to the skin, causing herpes zoster (also called shingles). Shingles generally affects the elderly and people with immunodeficiency.

Description: ViroReal® Kit VZV uses real-time PCR for the detection of the ORF29 gene coding for a single-stranded DNA-binding protein gene of VZV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

ViroReal® Kit VZV
OrdernumberReactionsChannel PathogenChannel IPCTarget
DHUV0225350FAMCY5ORF29
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
Product Features:
  • Amplification and detection: ORF29 of VZV
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit VZV is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional -  this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® VZV
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGM20V50FAM-ORF29
For research use only. Not for use in diagnostic procedures.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.