Background: The genus Pestivirus is composed of four important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus,” was identified. The BVD virus is a single strain, positive-sense RNA virus found throughout the world and causes bovine viral diarrhoea (BVD). BVD is a disease of cattle, which causes various clinical syndromes including diarrhea, mucosal disease, reproductive dysfunctions and/or hemorrhagic syndrome. Prenatal infection by BVD results in cattles permanently infected (BVD-PI). Such animals carry the virus throughout their lifetime and constantly secrete large numbers of viral particles.
Description: ViroReal® Kit BVD Virus uses one-step reverse transcription RT real-time PCR for the detection of 5´UTR of BVDV-1, BVDV-2 and BVDV-3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.
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Shipping Temperature: -20°C dry ice
- Amplification and detection: 5´UTR of BVD Virus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit BVD Virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.