Background: Batrachochytrium dendrobatidis, also known as the amphibian chytrid fungus, causes the disease chytridiomycosis in amphibians. It infects the keratinized skin of amphibians.

Description: MycoReal Kit Batrachochytrium dendrobatidis uses real-time PCR for the detection of the the ITS2 region of Batrachochytrium dendrobatidis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). For more detailed information see here.

MycoReal Kit Batrachochytrium dendrobatidis
OrdernumberReactionsChannel PathogenChannel IPCTarget
DVEF00311100FAMVIC/HEXITS2 region
DVEF00313100FAMCY5ITS2 region
DVEF0035150FAMVIC/HEXITS2 region
DVEF0035350FAMCY5ITS2 region
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: ITS2 region of Batrachochytrium dendrobatidis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: MycoReal Kit Batrachochytrium dendrobatidis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.


Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

MycoReal Batrachochytrium dendrobatidis
OrdernumberReactionsChannel PathogenChannel IPCTarget
RTGV939100FAM-ITS2 region
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal, ParoReal and ViroReal® Kits run with the same thermal cycling conditions.