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Product Manual BactoReal® Kit Clostridium difficile Download PDF
Material Safety Data Sheet BactoReal® Kit Clostridium difficile Download PDF
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Background:  Clostridium difficile is a Grampositive spore-forming anaerobic bacterium, commonly found in low concentrations in the intestine of clinically normal animals and humans. In higher concentration Clostridium difficile is an important cause of diarrhea in neonatal swine. Affected piglets may have dyspnea, abdominal distention, and scrotal edema. Diarrhea may not be present in all pigs affected. Pathogenic C. difficile strains produce multiple toxins. The best characterized are enterotoxin (Clostridium difficile toxin A causing fluid secretion into the gut lumen) and cytotoxin (Clostridium difficile toxin B), both of which may produce diarrhea and inflammation.

Description:  BactoReal® Kit Clostridium difficile uses real-time PCR for the detection of the Clostridium difficile toxin A (tcdA) gene and Clostridium difficile toxin B (tcdB) gene of C. difficile. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Clostridium difficile
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB04213 100 FAM, VIC Cy5 tcdA/tcdB
DVEB04253 50 FAM, VIC Cy5 tcdA/tcdB
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: tcdA gene and tcdB gene of Clostridium difficile
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Clostridium difficile is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional -  this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Clostridium difficile
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV920 100 FAM, VIC Cy5 tcdA/tcdB
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.