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Product Manual BactoReal® Kit Coxiella burnetii Download PDF
Material Safety Data Sheet BactoReal® Kit Coxiella burnetii Download PDF
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Background:  Coxiella burnetii (C. burnetii) is a Gram-negative, obligate intracellular, zoonotic bacterial pathogen, which is the causative agent of Q fever (Coxiellosis). The disease is spread through inhalation of a spore-like cell variant or close contact with body fluids. Cattle, sheep and goats represent the main animal reservoir.Seropositivity of ruminants for C. burnetii is associated with abortion, stillbirth and fertility problems. The high resistance to standard disinfectants and environmental influences renders disease control difficult.

Description:  BactoReal® Kit Coxiella burnetii uses real-time PCR for the detection of the IS1111 insertion sequence of Coxiella burnetii. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Coxiella burnetii
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05813 100 FAM Cy5 IS1111 insertion
DVEB05853 50 FAM Cy5 IS1111 insertion
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: IS1111 of Coxiella burnetii
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Coxiella burnetii is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Coxiella burnetii
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV231 100 FAM Cy5 IS1111 insertion
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.